Looking at the pinout, I would think it will work on an older 26 pin GPIO Pi.
If your worried about clearance issues you could use one of these to raise it up.
and these should work with that extra height.
But they appear to be missing some nuts for the threaded ends? You could just screw 4 of the stock standoffs to the ends and use them as a stand / feet.
One will line up for sure, not sure about the others though. I’m so used to all the 40 pin Pi’s having the mounting holes in the same place I forgot that the older ones have things shifted around a bit. Sorry about that.